Toxicity Test With Bslt (Brine Shrimp Lethality Test) Method of Notopanax fruticosum (L.) Miq Leaves

Abstract

Nothopanax fruticosum (L.) Miq is a plant from the Araliaceae family. The leaves of Nothopanax fruticosum (L.) Miq have a very strong antioxidant with the potential as an initial screening in toxicity assay. The research aimed to determine the LC50 value of Nothopanax fruticosum (L.) Miq leaf extract against shrimp larvae of Artemia salina Leach. The leaves were extracted using 96% ethanol by maceration. The ethanol extract was made with the concentrations of 25, 50, 250, 500, 1000, 3000 and its comparator used a sea water negative control. Each concentration consisted of 10 larvae with 3 replications. The observations were made after 24 hour-testing by counting the number of dead shrimp larvae. The LC50 value was calculated using probit analysis method. The results showed that the LC50 value of Nothopanax fruticosum (L.) Miq leaf extract was 66.07 μg / mL ± 12.16 μg / mL. This indicated that the leaf extract had a strong potential for toxicity to Artemia salina Leach shrimp larvae because the LC50 value was <1000 ppm.