Determination of Phenol and Flavonoid Content and Antioxidant Activity From Fraction of Ethyl Acetate of Siam Weed Leaves

Zainal Abidin; Andi Muflihunna; Sukmawati Sukmawati; Intan Saputri Amdad; Mardina Halik; Nisra Azis; Muzzakir Baits

Zainal Abidin
Andi Muflihunna
Sukmawati Sukmawati
Intan Saputri Amdad
Mardina Halik
Nisra Azis
Muzzakir Baits

Keywords: Phenol, flavonoid, antioxidant, ethyl acetat, FRAP

Abstract

Siam weed leaves (Chromolaena odorata L) are tradicionally used as a medicine, such as to treat the wound, antioxidant, painkillers (analgesic), feverlowering (antipyretic), and antibacterial. The leaves contain several main compounds such as tannins, phenols, flavonoids, saponins, and steroids. This research aimed to determine the phenol, flavonoid content and antioxidant activity from ethyl acetat fraction of siam weed leaves. The extraction was done by maceration using 96% ethanol and then fractionated by ethyl acetat. The fraction obtained was analyzed to determine of phenol and flavonoid content then antioxidant activity by the Uv-Vis spectrophotometry at the maximum wavelength. The results of this research that phenol and flavonoid content were 102,345 mgGAE/g fraction and 15,207 mgQAE/g fraction respectively, beside that this fraction analyzed its antioxidant activity by using Ferric Reducing antioxidant power (FRAP) method and showed that its antioxidant activity was 64,686 mgQE/gram fraction.