Delayed Type Hypersensitivity Study of Banana Peel (Musa paradisiaca L. Var Sapientum) Ethanol Extract in Wistar Rat

Abstract

Immunomodulators are compounds that are able to normalize the immune system in certain situations where the body is unable to normalize its immune system. This study aims to prove the immunomodulatory activity of ethanol extract of Banana peel (Musa paradisiaca L. Var Sapientum) with Delayed Type Hypersensitivity parameters. This study used male rats (Rattus norvegicus) which were divided into 5 groups. Group I was the normal group (without treatment), group II (induced SDMD 10% v/v), groups III, IV and V (treatment groups) were given extracts at doses of 100 mg/kg BW, 300 mg/kg respectively. BW, and 500 mg/kg BW. The test preparation was given orally for 7 days. On the 3rd and 7th days the test animals were induced with 10% v/v sheep red blood cells (SRBC) intraperitoneally and intraplantarly. Observations were made by measuring changes in the volume of the rats' legs at the 4th, 24th and 48th hours using a plethysmometer. Research data was processed statistically using the Kruskal-Wallis method followed by the Mann-Whitney test. Based on the research results, it can be concluded that extract doses of 100 mg/kg BW, 300 mg/kg BW, and 500 mg/kg BW can provide an immunomodulatory effect