Irritation Test of Seaweeds (Eucheuma cottonii. Doty) Powder Cream Formulation as Sunscreen In Vivo
Abstract
Over sunlight exposure in long periode may cause skin damage, premature aging and skin cancer possibilities. Sunsreen can protect the skin from negative effect of sunlight, it can reduce the negative effect of UV radiation. The objective of this research is making suncreen cream from seaweed powder Eucheuma cotoni.Doty by reducing the particle dimension, irritation test and set SPF value by in vivo way. This research begins with milling and seaving of seaweed particle through the process of cutting and grinding using a ball mill. Produced seaweed powder with size nano micrometer, and its characterized the particle size, polydispersity index, and morphology of the particles. The particle size is 532,73 ± 34,28 nm, and polydispersity index is 0,29 ± 0,07. Formulation using water-in-oil base cream consisted of cetomacrogol 1000, propylene glycol, VCO, and cetostearyl alcohol. Cream evaluation included organoleptic, pH, viscosity, irritation test and determine SPF value in vivo. Based of the result evaluation, the formula cream consisted of 1% cetomacrogol 1000, 15% propilen glikol, 56% VCO and 3% cetostearyl alcohol was recomended. The result of skin irritation test on rabbit’s back skin was negative. The first step SPF value was determined of Minimal Erythema Dose (MED). The minimal erythema dose (MED) that could cause erythema in albino rabbits of New Zealand strain 1,494 J/cm2. The SPF value of in vivo test is 3,36.
