Phytochemical Screening and Alphaglucooxidase Enzyme Inhibitory Activity Test of Secang Wood (Caesalpinia sappan L.) Ethanol Extract

Abstract

It is known that secang wood (Caesalpinia sappan L.) is a part of the Secang plant which is widely used to treat various diseases, including diabetes. Based on previous research, Secang wood contains flavonoids which have the potential to inhibit the α-glucosidase enzyme. The research aims to determine the potential of ethanol extracted by meeration using 96% ethanol. Inhibition of activity was tested using an ELISA (Enzyme-Linked Immunosorbant Assay) reader which was measured at a wavelength of 405 nm with acarbose as a comparison. The results of the research show that the ethanol extract of Secang wood has inhibitory activity, including it in the active category based on the % inhibition value obtained at 83,63%. Meanwhile, acarbose has inhibitory activity, including it in the very active category with a %inhibition value of 79%.