TLC Bioautography Analysis of Secang Wood Extract (Caesalpinia sappan L.) Against Propionibacterium acnes and Staphylococcus epidermidis Bacteria

Abstract

Secang wood (Caesalpinia sappan L.) is one of the plants that is widely used in traditional medicine in Indonesia. Secang wood is known to have several benefits, including its ability to treat several infectious diseases caused by bacteria. The aim of this research is to determine the class of active compounds that have antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis using TLC Bioautography analysis. The TLC results showed that the ethanol extract of secang wood contains alkaloid, flavonoid and phenol compounds. Bioautography TLC results show that the ethanol extract of secang wood has the potential for good antibacterial activity against the bacteria Propionibacterium acnes and Staphylococcus epidermidis with Rf values of 1: 0.92, Rf 2: 0.78, Rf 3: 0.65, Rf 4: 0.50, Rf 5: 0.38, Rf 6: 0.23 and Rf 7: 0.09 for Propinibacterium acnes. And the values of Rf 1: 0.65, Rf 2: 0.50, Rf 3: 0.38, Rf 4: 0.23 and Rf 5: 0.09 for Staphylococcus epidermidis.