Protein Level of Aedes Aegypti Larvae

Abstract

This research is aimed to determine the protein content of Aedes aegypti larvae. In the research method, the following procedures are as follows; the larval protein extraction, the fractionation using the ammonium sulfate at saturation levels of 0-30% (S2), 30-40% (S3), 40-60% (S4), and 60-80% (S5), 80 -100% (S6), the dialysis using the buffer solution B and C, determination of protein by using the Lowry method, protein purification using ion-exchange chromatography, and protein molecular weight measured by the SDS page. The research results showed the protein content of Ae. aegypti larvae extract; the crude extract sample (S1) contains only carbonic anhydrase, the 0-30% fraction (S2) contains trypsin inhibitor, the 30-40% fraction (S3) contains a-lactalbumin, trypsin inhibitor, carbonic anhydrase and ovalbumin, fraction 40-60% (S4) contains a-lactalbumin, trypsin inhibitor, carbonic anhydrase, ovalbumin and albumin, fraction 60-80% (S5) without protein content, fraction 80-100% (S6) contains trypsin inhibitor.