Antibacterial Activity Test on Horse Milk from Bima Regency, West Nusa Tenggara Against Bacteria Agar Diffusion Test

Abstract

This research aimed to determine the antibacterial activity of Sumbawa horse milk samples by agar diffusion methode. The screening result of antibacterial activity of Sumbawa horse milk samples at the concentrations of 0.8% possess the activity against the bacteria Bacillus subtilis, Vibrio cholerae, Staphilococcus aureus, and Escherchia coli. The test of minimum inhibitory concentration used the concentration of 0.4%, 0.8%, 1.6%, 3.2% and 6.4% against the bacteria Bacillus subtilis, Vibrio cholerae, Staphylococcus aureus, and Escherchia coli obtained the minimum inhibitory concentration value of the 0.8%. The test result of the minimum kill consentration against the bacteria Bacillus subtilis, Vibrio cholerae, Staphilococcus aureus, and Escherchia coli obtained the value 1.6% on the bacteria Escherchia coli and Staphylococcus aureus and 3.2% on the bacteria Bacillus subtilis and Vibrio cholerae. The test result of antibacterial activity of Sumbawa horse milk samples at the concentration of 0.8%, 1.6%, 3.2% 6.4% and 12.8% were able to inhibit the growth of the test bacteria with the greates inhibitory zone diameter at the concentration of 1.8% amounting to 14.65 mm againts the bacteria Escherchia coli