EXTRACT OF BLACK CUMIN SEEDS (NIGELLA SATIVA L.) AGAINST BACILLUS SUBTILIS

Armanto Makmun; Rahmawati Rahmawati; Desy Hardiyanti Endang Wihartati

Armanto Makmun Medical of Faculty, Universitas Muslim Indonesia, Makassar, Indonesia
Rahmawati Rahmawati Medical of Faculty, Universitas Muslim Indonesia, Makassar, Indonesia
Desy Hardiyanti Endang Wihartati Medical of Faculty, Universitas Muslim Indonesia, Makassar, Indonesia

Keywords: Nigella sativa, antibacterial activity, Bacillus subtilis, disk diffusion

Abstract

Background: Black cumin (Nigella sativa L.) contains active compounds with potential antibacterial properties. Bacillus subtilis was used as the test bacterium. Objective: To determine the antibacterial effect of ethanol extract of black cumin seeds (Nigella sativa L.) against Bacillus subtilis. Methods: The extract was prepared by ethanol maceration. The antibacterial test was conducted using the disk diffusion method with extract concentrations of 10%, 20%, 30%, and 40%, with tetracycline as the positive control and 10% DMSO as the negative control. The inhibition zones were measured in millimeters. Results: All extract concentrations produced inhibition zones, with the largest zone observed at the highest concentration, although smaller than that of tetracycline. Conclusion: Ethanol extract of black cumin seeds (Nigella sativa L.) has potential antibacterial activity against Bacillus subtilis, although its effectiveness is not yet optimal at concentrations below 40%.